Test : Osmotic Fragility/ Incubated Osmotic Fragility/ RBC fragility/ Red Cell Fragility.
Indications : Evaluate hemolytic anemia, especially hereditary spherocytosis, evaluate immune hemolytic states.
Physiology : Hereditary spherocytosis may be the result of an autosome dominant transmitted detect in red cell structural proteins. It is associated with a compensated or uncompensated hemolytic state, which is relieved by splenectomy. Most hereditary hemolytic anemia’s involve mutations of membrane structural proteins, the majority ode for abnormal spectrin molecules. A red cell protein 4.2 deficiency has been described. It has been reported recently found in individuals who have related anemia and whose red cells show osmotic fragility. Decreased osmotic fragility maybe seen with iron deficiency, other hemoglobinopathy, hemoglobin C disease, likely due to the target cell population, and is characteristic of thalassaemia. Osmotic fragility is increased in cases of malaria infestation.
Normal Range : Hemolysis begins 0.45%. hemolysis complete 0.35%
Interpretation : Percent hemolysis is determined using optical density measurements.
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What is the range of pH to be maintained in SAGM Solution for storage of Red Blood Cells in blood bank.