New Methods to Screen Alzheimer's Drugs

Researchers at UC Davis have found a mechanism by which the amyloid protein damages the brain- a finding that could lead to new ways to screen drugs for Alzheimer's

New ways to screen drugs for Alzheimer's and similar diseases have been developed by researchers at UC Davis through a mechanism by which the amyloid protein damages the brain.

The researchers found that brain cells exposed to a form of the amyloid beta protein, the molecule linked to Alzheimer's disease, become stiffer and bend less under pressure.

Alzheimer’s disease - Causes, Symptoms, Diagnosis, Stages, Types, Prevention, Prognosis & Treatment
Alzheimer's disease is a progressive neurodegenerative disease affecting memory and thinking and making the person increasingly dependent on others.

The team, led by Gang-Yu Liu, professor of chemistry, and Lee-Way Jin, associate professor of pathology and a researcher at the UC Davis Alzheimer's Disease Center, used a cutting-edge microscope to measure how cells respond to physical pressure.

The microscope, located at UC Davis' Spectral Imaging Facility, combines an atomic force microscope and a confocal microscope. It is one of a handful in the United States and one of the most advanced of its type, Liu said.

An atomic force microscope uses a fine needle to visualize the features of a surface with exquisite resolution and precision; it is used more often in materials science than in cellular biology.

Alzheimer's Drug is Both Safe and Effective, Study Shows
Memantine-a drug used to treat Alzheimer's disease helps patients without causing serious side effects and a new study shows how.

A confocal microscope can view living cells in culture media and in three dimensions.

The team put a glass microbead on the tip of the AFM needle and used it to press down on living cells.

In Normal Aging Process, Proteins Linked With Alzheimer's Clump
Many of the proteins present as minor components of Alzheimer's and other neurodegenerative diseases actually clump together as a normal part of aging in healthy individuals

By measuring the forces required to squeeze the cell under the bead, they could calculate the stiffness of both the cell membrane and the cell contents.

"This is a simple method for measuring the stiffness of a cell - like pushing down on a spring," said Liu.

Early Prediction of Alzheimer's Possible With Discovery of New Brain Passage
UC Irvine researchers have identified for the first time in humans a long-hidden part of the brain called the perforant path, which may allow for early diagnosis of Alzheimer's disease.

The "squeeze" test could be used as a screening method for potential drugs for Alzheimer's and other diseases, predicted the researchers.

Normal brain cells are the "squishiest" among the cell types they have tested with the technique, said Liu.

The cells readily deform under pressure, but recover. At the other end of the scale, skin cells (keratinocytes) are very stiff and resistant to pressure, but shatter under stress.

The findings are published this month in the Proceedings of the National Academy of Sciences.

Source-ANI