Monkeypox Virus in Testes of Non-human Primate Survivors Detected

Monkeypox virus may be shed into semen during both acute and convalescent stages of the disease in crab-eating macaques.

For the first time, researchers have discovered monkeypox virus in the testes of macaques during the acute phase of infection.

In addition, the team found preliminary evidence of persistent infection in two animals that survived challenge with the virus. Their results highlight the potential for sexual transmission of the virus in humans.

Monkeypox - Causes, Symptoms, Signs, Diagnosis, Treatment, Prevention
Monkeypox infection is a zoonotic viral infection that usually lasts for 3 weeks. It is caused by the monkeypox virus and is characterized by rash or pox-like symptoms.

‘The research team found preliminary evidence of persistent monkeypox virus infection in two convalescent crab-eating macaques that survived challenge with the virus.’

The ongoing 2022 monkeypox outbreak has been linked to sexual contact in patients with laboratory confirmed infection. As the virus can be transmitted through direct contact with bodily fluids and skin lesions, understanding the biology of monkeypox infection of the testes, and virus shedding in semen, has substantial public health implications.

Prevalence of Monkeypox Among Macaques

Investigators at the U.S. Army Medical Research Institute of Infectious Diseases, or USAMRIID, performed a retrospective analysis of monkeypox virus infection in archival tissue samples from crab-eating macaques—a widely used nonhuman primate model for studying the disease and evaluating the efficacy of medical countermeasures, such as vaccines and treatments, against monkeypox.

“We examined tissue samples obtained during both the acute phase of the disease, when infection is at its peak, and the convalescent phase, when infection is gradually subsiding,” explained senior author Xiankun (Kevin) Zeng, Ph.D., of USAMRIID. “We detected monkeypox virus in interstitial cells and seminiferous tubules of the testes, as well as the epididymal lumina, which are the sites of sperm production and maturation.”

Using histological analysis to microscopically analyze the disease course in tissue samples, the USAMRIID team discovered that while monkeypox virus was cleared from most organs—and from healed skin lesions—during convalescence, it could be detected for up to 37 days post-exposure in the testes of the macaques.

Test Your Knowledge on Monkeypox
Introduction:

Monkeypox is a rare viral infection caused by the monkeypox virus, which belongs to the same family of smallpox. It typically occurs in central and western Africa. Sometimes, small clusters or isolated infections are diagnosed in other countries.

Important Facts on Monkeypox
With the rapid increase in the 2022 outbreak of monkeypox cases, the following facts may help ease the alarming rates of misconceptions and concerns that have coursed among public.

Monkeypox does not spread because of monkeys. The name originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. There are two forms of monkeypox, a milder west African strain and a more severe central African, or Congo strain. The current outbreak involves the west African strain, although not all countries have looked into this.

Anyone can spread monkeypox through contact with body fluids, monkeypox sores, or shared items (such as clothing and bedding) that have been contaminated with fluids or sores of a person infected with monkeypox.

USAMRIID investigators, led by Zeng, had previously demonstrated that Ebola, Marburg, Nipah, and Crimean-Congo hemorrhagic fever viruses can persist in certain organs of nonhuman primate survivors where the immune system is suppressed. These immune privileged sites, which are similar in humans, include the eyes, brain, and testes.

Experts say Monkeypox Infections Slowing in US
In the United States, there have been fewer new cases of monkeypox recorded recently. Experts say it is too soon to tell if the trend will continue, media reports said.

While close contact through sexual activity has been associated with the spread of monkeypox virus in the current global epidemic, it was unclear whether the virus replicated in the testes or was transmitted via semen.

“It seems plausible, therefore, that human transmission in convalescent male patients might occur via semen.” The authors also noted that persistent virus may be cleared over time.

Because this was a retrospective study using archival tissues, virus isolation in semen was not possible, said Jun Liu, Ph.D., the paper’s first author. Further studies are now needed to understand the origins, dynamics and implications of viral DNA shed in semen, as well as to confirm whether semen from convalescent monkeypox patients contains infectious virus—especially after skin lesions heal.

In addition, according to the authors, the crab-eating macaque model may not fully reflect monkeypox in humans. The animals demonstrate a more severe and lethal disease than that observed in humans, and the incubation period in the animals is shorter. Furthermore, this study used samples from animals exposed to different viral isolates than the strain currently circulating.

USAMRIID has decades of experience working with monkeypox and related high-threat pathogens. Among the Institute’s many contributions to the field are a novel respiratory model for monkeypox that closely approximates human exposure and clinical disease; the Phase 3 clinical trial supporting U.S. Food and Drug Administration licensure of the JYNNEOS (Bavarian Nordic) vaccine for preventing smallpox and monkeypox in humans; and initial screening and preclinical efficacy testing of TPOXX (SIGA Technologies), the first drug approved for treatment of smallpox. USAMRIID continues to develop monoclonal antibodies and other approaches to protect against orthopoxviruses.

Source-Eurekalert