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Two New Genes Linked to Development of Rare Neuroendocrine Tumors

by Anjali Aryamvally on July 22, 2017 at 5:39 PM
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Highlights:


Pheochromocytomas (PCC) and paragangliomas (PGL) are rare neuroendocrine tumors. Many genes whose alterations or mutations confer hereditary susceptibility to develop these tumors are those that are involved in the Kreb's cycle (cell respiration pathway). A team of researchers from the Spanish National Cancer Research Center have identified rare pathological variations in known PGL/PCC susceptible genes and also two new candidate genes that confer hereditary susceptibility. The study was published in the journal Clinical Cancer Research.

Pheochromocytoma and Paraganglioma


Pheochromocytoma (PCC) and paraganglioma (PGL) are rare tumors that form from neuroendocrine cells. They are both generally benign (do not grow a.k.a cancerous) but rarely they do undergo metastasis (spread to other parts of the body). PCC develops in the adrenal gland and PGL in certain nerve cells. Both tumors result in episodic or persistent high blood pressure.


Both PGL and PCC are not genetic in origin but the generally benign tumors becoming cancerous and spreading to other parts of the body is associated to mutations in genes involved in the cell respiration cycle. The fact that up to 40% of individuals with PCC and/or PGL have a hereditary background due to germ line mutations affecting one of thirteen susceptibility genes makes these tumors have a stronger hereditary component compared to other tumors.

Study lead, Alberto Casc�n, mentioned "The identification of mutations in the Krebs cycle genes is especially important given that the patients harboring them have a greater likelihood of developing metastasis"

‘GOT2 and IDH3B genes possibly linked to hereditary predisposition to paragangliomas and pheochromocytomas.’

Basis of The Study

Nearly half of the genes linked to development of PGG and PCC were linked to genes that play a role in the Kreb's cycle. Variations in genes that code for SDH, FH and MDH2 leads to accumulation of metabolites that lead to epigenetic (combination of environmental and genetic) changes in the genome. The team hypothesized that if tumors which had the same epigenetic changes but the already known genes were not mutated, then there must be mutations in other Kreb's cycle genes.

49 tumors from patients that had the same epigenetic changes and did not show alterations in the thirteen known PCC/PGG susceptibility genes were analyzed. Exome sequencing revealed two very rare pathological variations in known PCC/PGL susceptibility genes (SDHC and IDH1).

The study also identified two new candidate genes tat could possibly be involved in the hereditary predisposition to PCC and PGL. One is a germ line mutation in the GOT2 gene and the other IDHB3 gene both encoding enzymes for the Kreb's cycle.

The study strengthens the evidence for the relevance of Kreb's cycle genes in the development of pheochromocytomas and paragangliomas and also indicates that there may be several undiscovered genes that may be involved.

Study Significance

The epigenetic changes in the genome as a result of the gene mutations lead to dis-regulation of the enzyme levels. If all the susceptibility genes are identified, the epigenetic changes may be rewritten. In this case the DNA becomes hyper-methylated (additional methyl groups are added, thereby silencing or altering gene function). To bring back normal function the methyl groups may be removed.

Identifying all susceptibility genes also means that the a person affected with these neuroendocrine tumors can track the tumor and eliminate the risk of metastasis.

References:

  1. Remacha, L., Comino-M�ndez, I., Richter, S., Contreras, L., Curr�s-Freixes, M., Pita, G., . . . Cascon, A. (2017). Targeted exome sequencing of Krebs cycle genes reveals candidate cancer predisposing mutations in pheochromocytomas and paragangliomas. Clinical Cancer Research. doi:10.1158/1078-0432.ccr-16-2250
  2. Pheochromocytoma and Paraganglioma-Patient Version - (https:www.cancer.gov/types/pheochromocytoma)
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