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Curative �Shock and Kill' Strategy for HIV Treatment, Could Backfire

by Dr. Meenakshy Varier on December 6, 2016 at 3:31 PM
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Highlights

A proposed curative strategy for HIV known as "shock and kill" can actually cause more harm if the virus if lying dormant in the brain.


While combination drug treatments have become successful in controlling HIV infection, the goal of totally curing patients has so far been hindered by HIV's ability to become dormant for long periods of time.

‘Extra caution should be applied when looking for ways to remove HIV reservoirs and eradicate the virus from the body.’

A new study conducted on HIV's close cousin, simian immunodeficiency virus (SIV), in macaques, shows that curative strategy could backfire.

Shock and Kill

The "shock and kill," wake up the dormant viruses using latency-reversing drugs, forcing it to reveal itself and making them vulnerable to the patient's immune system.

The aim of such a strategy is to wipe out majority of infected cells, in combination with antiretroviral medicines.

This strategy could be harmful if the HIV reservoirs exist in the brain.

But a study of macaques with SIV, has shown that such a strategy could cause potentially harmful brain inflammation. The study was published in the journal AIDS.

"The potential for the brain to harbor significant HIV reservoirs that could pose a danger if activated hasn't received much attention in the HIV eradication field," says Janice Clements, Ph.D., professor of molecular and comparative pathobiology at the Johns Hopkins University School of Medicine. "Our study sounds a major cautionary note about the potential for unintended consequences of the shock-and-kill treatment strategy."

HIV research have focused on prevention and developing antiretroviral therapies to keep the virus in check without eradicating it, says Lucio Gama, Ph.D., assistant professor of molecular and comparative pathobiology at Johns Hopkins and the lead author of the new study.

Current Study

The researchers treated three pig-tailed macaque monkeys that were infected with SIV with antiretrovirals for more than a year.

Two of the three macaques were given ingenol-B, a latency-reversing agents, to "wake up" the virus.

"We didn't really see any significant effect," Gama says, "So we coupled ingenol-B with another latency-reversing agent, vorinostat, which is used in some cancer treatments to make cancer cells more vulnerable to the immune system."

The macaques also continued their course of antiretrovirals throughout the experiment.

Encephalitis

After a 10-day course of the combined treatment, the macaques which was not given the curative treatment remained healthy. The others developed symptoms of encephalitis, or brain inflammation and blood tests were positive for SIV infection.

The researchers humanely killed the macaques, when their condition worsened and carefully removed the blood from their body.

Testing revealed SIV was still present in the brain, but only in one of the regions analyzed-the occipital cortex, which processes visual information. The affected area was very small .

Points to Remember

The results on macaques with SIV may not apply to humans with HIV.

The encephalitis could have been transient and resolved on its own.

The results signal a need for extra caution in exploring ways to remove HIV reservoirs and eradicate the virus from the body.

Source: Medindia

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