Liver MicroRNAs Needed for Survival by Hepatitis C Virus

The way in which hepatitis C virus survives in the liver has been discovered by researchers, thus helping scientists understand why a new antiviral drug appears to be effective against the virus.

Scientists at the University of North Carolina at Chapel Hill, working with colleagues from the University of Colorado, have found for the first time how the hepatitis C virus hijacked a small RNA molecule that regulates gene expression in human liver cells to ensure its own survival.

MicroRNAs are involved in regulating the expression of genes in cells, usually by blocking the production of key proteins or by destabilizing the messenger RNAs that encode the cell's proteins as it grows and divides. ormally they act by down regulating gene expression.

The research team found that the binding of a prominent microRNA in liver cells, called miR-122, to the viral RNA results in its stabilization, promoting efficient replication of the virus genome in the liver and supporting the virus' lifecycle.

"The hepatitis C virus has done two very interesting things with miR-122," said Stanley M. Lemon, MD, professor of medicine and microbiology and immunology and a member of UNC Lineberger Comprehensive Cancer Center and the Center for Translational Immunology.

"First, it has evolved a unique relationship with a key regulator, since miR-122 represents about half of all microRNAs present in the liver. Second, the virus has usurped a process that usually downregulates gene expression to upregulate the stability of its RNA and expression of viral proteins needed for its lifecycle.

"It's a classic example of how viruses subvert normally beneficial functions of the cell to their own nefarious purposes," stated Lemon.

Work by Dr. Lemon and his colleagues in 2005 helped to demonstrate that miR-122 was required for hepatitis C to replicate itself, but the mechanism was not understood.

Now the UNC research team has shown how it works, which helps to explain how a new experimental antiviral drug target the virus.

The drug, called an "antagomer", binds to miR-122 and sequesters it in the liver and thus destabilizes the viral genome, accelerating its degradation in the liver.

Results of the study have been published online this week in the journal Proceedings of the National Academy of Sciences.

Source: ANI