Hematology Test

Test : Auto hemolysis Test


Indications : This test is used to diagnose hereditary spherocytosis, spontaneous hemolysis, RBC enzyme deficiency and hemolytic anemia.

Physiology : This test measures the degree to which patients red cells lyse without additives, with glucose and with ATP (Adenosine Tri-phosphate).

Interpretation : Glucose by itself can induce hemolysis in auto hemolysis test in patients with hereditary spherocytosis. Glucose-6-Phosphate dehydrogenase deficient RBC’s have increased auto hemolysis, which corrects significantly with glucose or ATP. Pyruvatekinase deficiency has increased auto-hemolysis, which does not correct or get aggravated with glucose but does correct toward normal with ATP.

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Related Tests : Glucose-6-Phosphate dehydrogenase Osmotic Fragility, Red Blood Cell Morphology, RBC enzyme deficiency.

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