Sputum Test

Test : Pulmonary aspergillosis


Indications : Aspergillosis test is done when respiratory symptoms resembling those of aspergillosis are seen in those who have a weak immune system such as those with cancer, AIDS, leukemia, those who underwent organ transplant, chemotherapy, or other conditions or medications.

Sputum collection is done early in the morning. The patient must refrain from eating or drinking before the test. It is also essential to brush teeth and rinse mouth well prior to the test. Avoid using antiseptic mouthwash. Sputum may be brought up by deep coughing (expectorated) or it may be or induced with the help of sterile saline or glycerin aerosol. The coughed up sputum is collected into a sterile cup provided by the laboratory.

A small part of the sputum is stained with a dye and observed through the microscope for the presence of aspergillus filaments.

The sample is then placed in growth medium and the mold is allowed to grow for a period of time.

Physiology : Aspergillosis is a lung infection caused by a fungus Aspergillus, which is seen growing among decaying vegetation. Symptoms of aspergillosis include – chest pain, fever and shortness of breath.

Types of Aspergillosis:

• Pulmonary aspergillosis - An allergic reaction to the fungus more common in those with asthma or cystic fibrosis.

• Aspergilloma - a fungal ball-like growth that usually occurs in an place that has formerly been affected by a lung disease such as TB or lung abscess.

Normal Range : Negative

Interpretation : The presence of aspergillus filaments in the culture dishes confirms the diagnosis of aspergillosis.

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