Alterations in ‘Inflammation-Control’ Kidney Genes Causes More Organ Failure

Kidney damage often sets off a slew of complications in patients, spreading organ failure like wildfire throughout their bodies. Now, researchers at Johns Hopkins say they have evidence in mice that this deadly progression-at least to the lungs-may be due to genetic alterations in kidney-based genes that sabotage inflammation- control and send toxic signals to healthy organs. The signals convince these organs to react as if they, too, are damaged.

The study, led by Dmitry N. Grigoryev, M.D., Ph.D., instructor in the Division of Clinical Immunology at the Johns Hopkins University School of Medicine, identifies 109 inflammatory genes that change activity with remarkable similarity in kidneys and lungs after kidney damage due to disease or injury.

'What we hope is that these genes will eventually provide new targets to prevent wider organ failure before it occurs,' Grigoryev says.

The research team focused on collateral damage to the lungs, since this organ is one of the first to fail in many patients with kidney damage. This complication is also extraordinarily deadly, killing about 80 percent of those who experience it.

Scientists have previously suggested that the lungs might be picking up chemicals that injured kidneys leak into the bloodstream. 'The lung is a huge filter. Whatever signals the kidneys are pumping out, the lung sees. The lung doesn’t know it’s healthy-these signals make it think it’s sick,' says Grigoryev.

To identify those chemical signals, he and his team experimentally cut off blood flow to kidneys in one group of test mice for up to 60 minutes, irreparably damaging the organs.

Other animals had sham operations that didn’t damage the kidneys.

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The researchers then took tissue samples from the animals’ kidneys and lungs, some at 6 hours and some at 36 hours after the operations, and examined more than a thousand genes linked to the production of chemicals produced when tissues are inflamed by disease or other damage.

Comparing activity levels of genes in animals with damaged kidneys to those without, the researchers found that several inflammatory genes, including Cd14, Socs3, Saa3, Lcn2, and Il1r2, increased activity in both organs. Further tests showed that this rise appeared to set off a cascade of chemical signaling that causes the kidney and circulating white blood cells to release inflammatory proteins.

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The researchers found especially large amounts of two of these proteins, interleukin-6 (IL-6) and interleukin-10 (IL-10), in the bloodstream of mice with damaged kidneys. The results, published in the Mar. Journal of the American Society of Nephrology, provided a detailed signature of kidney injury in kidney and lung genes. Grigoryev and his colleague plan to extend this research by creating genetically modified mice that aren’t able to make IL-6 and IL-10, then testing how their lungs respond to kidney injuries. Eventually, he notes, drugs that target these and other inflammatory proteins produced when kidneys are injured may prevent lungs and other organs from suffering collateral damage.

This study was supported by grants from the National Institutes of Health Acute Lung Injury SCCOR, the Mary Beryl Patch Turnbull Scholar Program, and the National Kidney Foundation.

Source-JOHN HOPKINS
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