Chocolate Yummier With New Genome Sequence

A variety of gene families that may bring about a higher quality chocolate have been indentified by an international team of scientists.

The team, lead by Claire Lanaud of CIRAD, France, with Mark Guiltinan of Penn State, sequenced the DNA of a variety of Theobroma cacao- considered to produce the world's finest chocolate.

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This variety of Theobroma cacao, Criollo, was domesticated about 3,000 years ago in Central America, and it is one of the oldest domesticated tree crops.

"Fine cocoa production is estimated to be less than five percent of the world cocoa production because of low productivity and disease susceptibility," said Guiltinan.

Since the farmers who grow fine cacao earn more than others, increasing the productivity and ease of growing cacao can help to develop a sustainable cacao economy.

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The team has identified a variety of gene families that may have future impact on improving cacao trees and fruit either by enhancing their attributes or providing protection from fungal diseases and insects that effect cacao trees.

"Our analysis of the Criollo genome has uncovered the genetic basis of pathways leading to the most important quality traits of chocolate-oil, flavonoid and terpene biosynthesis," said Siela Maximova of the Penn State, and a member of the research team.

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"It has also led to the discovery of hundreds of genes potentially involved in pathogen resistance, all of which can be used to accelerate the development of elite varieties of cacao in the future," said Maximova.

Guiltinan and colleagues are interested in specific gene families that could link to specific cocoa qualities or disease resistance. They hope that mapping these gene families will lead to a source of genes directly involved in variations in the plant that are useful for acceleration of plant breeding programs.

The researchers have identified two types of disease resistance genes in the Criollo genome. They compared these to previously identified regions on the chromosomes that correlate with disease resistance-QTLs-and found that there was a correlation between many the resistance genes' QTL locations.

The team suggested that a functional genomics approach, one that looks at what the genes do, is needed to confirm potential disease resistant genes in the Criollo genome.

Hidden in the genome the researchers also found genes that code for the production of cocoa butter, a substance highly prized in chocolate making, confectionary, pharmaceuticals and cosmetics.

Most cocoa beans are already about 50 percent fat, but these 84 genes control not only the amounts but also quality of the cocoa butter.

Other genes were found that influence the production of flavonoids, natural antioxidants and terpenoids, hormones, pigments and aromas. Altering the genes for these chemicals might produce chocolate with better flavors, aromas and even healthier chocolate.

The study was reported in the journal Nature Genetics.

Source-ANI