IgM ELISA can be used as primary diagnostic tool followed by polymerase chain reactions for Nipah virus (NiV) detection.
Overview of ELISA
Enzyme Linked Immunosorbent Assay (ELISA) is a safe, sensitive, specific, and affordable diagnostic tool that can be used during screening of large-scale epidemiological investigations. The development and evaluation of IgM and IgG ELISA for screening serum samples of NiV in suspected cases would also help in planning public health interventions, ICMR scientists have underlined in a study. The sensitivity, specificity, and cross-reactivity of the assays were evaluated using NiV IgM, IgG positive, negative human sera and measles, mumps, rubella, Crimean-Congo haemorrhagic fever, Kyasanur forest disease IgM, IgG positive sera, respectively, the study noted.
Nipah Virus (NiV) | Hendra Virus - Causes, Symptoms, Consequences, Diagnosis, Treatment and Prevention
Nipah Virus (NiV) or Hendra virus is a deadly virus that spreads via contact with the saliva, urine, or excreta of fruit bats or pigs. Nipah outbreak is an emerging zoonosis which had resulted in fatal deaths in Kerala, India.
Nipah Virus (NiV) or Hendra virus is a deadly virus that spreads via contact with the saliva, urine, or excreta of fruit bats or pigs. Nipah outbreak is an emerging zoonosis which had resulted in fatal deaths in Kerala, India.
‘An IgM capture (MAC) ELISA and an indirect IgG ELISA were developed using Nipah virus (NiV) antigen to detect IgM and IgG antibodies against NiV in human sera.’
The scientists have also claimed that the developed anti-NiV IgM and IgG ELISAs have shown specificity of 99.28 per cent and sensitivity of 100 per cent compared to reference test from Centers for Disease Control and Prevention, USA. Assays demonstrated negative predictive value of 100 per cent and positive predictive value as 90 and 93.94 per cent for anti-Nipah IgM ELISA and IgG ELISA respectively with test accuracy of 99.33 per cent. Timely diagnosis of NiV is crucial for the management of cases, which could prevent further spread of infection in the community. IgM ELISA can be used as primary diagnostic tool followed by polymerase chain reactions, it claimed.
Source-IANS
Test Your Knowledge on Nipah Virus
Nipah Virus Infection
Nipah virus infection is a newly emerging zoonoses (infection transmitted from animals to humans) capable of causing serious illness in humans as well as animals. Prevention is key to reducing the transmission. Take this quiz to see how much you know about Nipah virus infection and learn some more interesting general and medical information about Nipah virus.
Nipah Virus Infection
Nipah virus infection is a newly emerging zoonoses (infection transmitted from animals to humans) capable of causing serious illness in humans as well as animals. Prevention is key to reducing the transmission. Take this quiz to see how much you know about Nipah virus infection and learn some more interesting general and medical information about Nipah virus.
New Nipah-Like �Langya Virus� Detected in China
Is there a new viral outbreak in China? nearly three dozen people in China have been infected by the Langya virus, likely to have jumped into humans from shrews.
Is there a new viral outbreak in China? nearly three dozen people in China have been infected by the Langya virus, likely to have jumped into humans from shrews.
Nipah Scare in Kerala: What Went Wrong?
People should be aware against trespassing into bat habitats. Also, avoid eating contaminated fruits or interacting with domestic animals without proper protection.
People should be aware against trespassing into bat habitats. Also, avoid eating contaminated fruits or interacting with domestic animals without proper protection.
