Hidden Role of Nervous System Cells Revealed

Distinct stimuli such as light touch or pressure activates spinal sensory neurons and the intensity of the stimulus reflects in the neuron's activity.

Everyday activity of cells within the spinal cord studied with an innovative technology revealed that astrocytes--cells in the nervous system that do not conduct electrical signals and were traditionally viewed as merely supportive--unexpectedly react to intense sensation. //

The new miniaturized microscope and related imaging methods, described by Salk Institute scientists on April 28, 2016 in Nature Communications, offer unprecedented insight into nervous system function and could lead to novel pain treatments for spinal cord injuries, chronic itch and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS).

Response to Stimulus Different from Cell to Cell
Contrary to what was once believed, there is a difference in the way each cell responds to stimulus, concludes a recent study.

‘Astrocytes cannot send electrical signals like neurons can, they generated their own chemical signals in a coordinated way during intense stimuli’

The spinal cord is crucial for sensing and responding to the world. Sometimes it even works independently from the brain, such as when your hand recoils from a hot stove before the sensation has fully registered. But it is unknown exactly how the cells within the spinal cord encode these and other feelings from the skin or internal organs.

In the new study, senior author Axel Nimmerjahn, an assistant professor in Salk's Waitt Advanced Biophotonics Center, and his team improved upon the miniaturized microscopes they first described back in 2008. The researchers' new version--which features numerous hardware and software improvements--enabled them to visualize changes in cellular activity in awake, roaming mice.

"For a long time, researchers have dreamed of being able to record cellular activity patterns in the spinal cord of an awake animal. On top of that, we can now do this in a freely behaving animal, which is very exciting," says first author Kohei Sekiguchi, a Salk researcher and PhD student at the University of California, San Diego.

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In the new work, the group found that distinct stimuli--such as light touch or pressure--activate different subsets of spinal sensory neurons. They also found that certain features, like the intensity or duration of a given stimulus, are reflected in the activity of the neurons.

To the team's surprise, astrocytes, traditionally thought to be passive support cells, also respond to stimuli (albeit differently than the neurons). Though the astrocytes cannot send electrical signals like neurons can, they generated their own chemical signals in a coordinated way during intense stimuli.

Neural Code of the Brain Decoded by Researchers
The brain cells were activated and their responses were measured using flashes of light. This is a new approach to read the signals of the brain.

Nimmerjahn is excited about this result because his group has a longstanding interest in understanding astrocytes and their roles in nervous system function and disease. These cells are increasingly appreciated as important players in how the nervous system develops and operates and could serve as promising new drug targets, he says.

"Not only can we now study normal sensory processing, but we can also look at disease contexts like spinal cord injury and how treatments actually affect the cells," says Nimmerjahn.

About Kyphosis
Kyphosis is a spinal disorder, which is derived from Greek word – 'kyphos' which means bowed or bent. Spinal curve of the backbone in the upper back causes bowing of the backbone. It also affects the lower back.

The team is now working to simultaneously record touch or pain-related activity in the brain and spinal cord using additional iterations of the miniaturized microscopes, which allow them to monitor and manipulate multiple cell types at even higher resolutions.

Source-Eurekalert