Nasal Vaccines: A Promising Alternative to Injections?

The nose, a portal to our bodies, filters air, scents, and pathogens. Within the nasal passage, the turbinates, bone structures resembling seashells, play a crucial role. Covered in mucus-secreting tissue and nerve endings, they warm and humidify inhaled air. While a hotspot for pathogen entry, the turbinates' proximity to the brain poses a challenge for immune system protection. A new study in Nature reveals a fascinating defense mechanism. Antibody-producing cells migrate to the turbinates during illness or vaccination, locally secreting antibodies to combat invaders (1^✔ ✔Trusted Source
Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues

Go to source). This discovery could lead to improved nasal vaccines and treatments for neurological, allergic, and autoimmune conditions.

During the coronavirus pandemic, when the virus was spreading across the globe, millions of people kept close tabs on the development of a COVID vaccine that could be administered as a nasal spray instead of an injection. The idea was not outlandish; after all, a flu vaccine and several others are already available through nasal sprays, which contain live but weakened viruses that generate local protection in the nasal conchae. These nasal vaccinations, however, are not effective in a single dose and must be followed up by a booster dose. Yet, scientists do not yet fully understand how these nasal vaccines work and why a booster is required.

To shed light on the matter, Prof. Ziv Shulman of Weizmann’s Systems Immunology Department and Jingjing Liu, a PhD student in his lab, decided to examine how immune system organs located near the nose and throat respond to nasal vaccinations. In humans these organs include the tonsils and the adenoids, collectively known as Waldeyer’s lymphatic ring, or tonsillar ring. In this new study, a team of researchers, led by Liu, used advanced imaging techniques to analyze the body’s immune response by imaging whole and intact immune system organs of mice, similar to those of humans.

The researchers discovered that when mice were given a nasal vaccination, a focused immune response was mounted by B cells, the immune system’s key producers of antibodies. These cells begin their journey as precursor B cells, some of which have the potential to identify pathogens. The scientists saw how cells located close to the mucous tissue that covers the nasal cavity identified the vaccine molecules and started to divide and differentiate rapidly. This differentiation process can be seen as a kind of biological specialization course, which ends when they become cells that secrete antibodies specific to the pathogen or become memory cells. The memory cells are stored for extended periods of time in case of future infection.

B Cells and Nasal Vaccines: A Behind-the-Scenes Look

B cells can secrete five types of antibodies. The researchers discovered that in response to a vaccine the B cells close to the nasal cavity change the identity of the antibody that they produce and start secreting antibodies that act as “gatekeepers” that specialize in passing from the inner mucous tissue into the nasal cavity. The next stage is for the B cells to move from their location close to the nasal cavity to tiny “training camps,” known as germinal centers, which are found in the immune system organs in that part of the body. Once there, they undergo “training” that includes changes to their genetic make-up and a meticulous selection process to ensure the survival of only those B cells that produce the antibodies effectively linked to the pathogen targeted by the vaccine.

The B cells are aided in this training plan by a certain type of T cell, which even plays a role in deciding which cells will survive in the end, but the researchers discovered that there are not enough of these T cells in the organs of the immune system in that part of the body to create an effective immune response. “The fact that there has to be a migration of T cells to the area explains why one dose of a nasal vaccine is not enough and a booster dose is required,” Shulman explains. “It is only after the second dose that enough of the required T cells are produced in order to turn the B cells into effective antibody producers and into memory cells.”

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The researchers believe that the scarcity of T cells is designed to prevent oversensitivity to harmless quantities of foreign objects in the air. “It is possible that this mechanism goes awry when people develop allergies and various autoimmune diseases, so understanding it could help develop new treatments for these conditions,” Shulman adds.

Discovering that the immune mechanism was activated in response to vaccines was only the starting point for an intensive search to determine where the antibody-secreting cells go after the differentiation process is completed. “We visualized an immune response in the nasal lymph nodes, but how is it translated into airway protection?” says Shulman. “We were surprised to discover the B cells in the nasal conchae, bone tissue that was not known to support an antibody-mediated immune response. This relocation to bone tissue is similar to what happens in bone marrow, and it is possible that this niche environment has other roles beyond the immune mechanism that we identified.”

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The new findings show that the antibody-secreting cells translocate themselves from the nasal lymph nodes to the mucus-producing glands in the nasal conchae, directly below their external layers of cells, and that they secrete their antibodies into these glands. This immune defense makes up for the blood antibodies’ inability to get to this gateway through the blood stream, and it is important not only in the context of viruses and other diseases: It also protects the brain and the many nerve endings in that part of the body, which are responsible for our sense of smell.

In addition to facilitating vaccine design, Shulman notes that this research has exposed “an entry point into a highly fortified target – antibody-secreting cells that have access to the central nervous system.” In the future it might be possible to make use of the antibody-secreting cells’ access to the olfactory nerves in order to design vaccines for neurological diseases.

Reference:

1. Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues - (https://www.nature.com/articles/s41586-024-07729-x)

Source-Eurekalert