A small fragment of the llama antibody known as nanobody discovered by scientists is capable of chasing out human cytomegalovirus (HCMV) as it hides away from the immune system.
A small fragment of the llama antibody known as nanobody discovered by scientists is capable of chasing out human cytomegalovirus (HCMV) as it hides away from the immune system. This then enables immune cells to seek out and destroy this potentially //deadly virus. Around four out of five people in the UK are thought to be infected with HCMV, and in developing countries this can be as high as 95%. For the majority of people, the virus remains dormant, hidden away inside white blood cells, where it can remain undisturbed and undetected for decades. If the virus reactivates in a healthy individual, it does not usually cause symptoms. However, for people who are immunocompromised - for example, transplant recipients who need to take immunosuppressant drugs to prevent organ rejection - HCMV reactivation can be devastating.
‘The approach could lead to a much-needed new type of treatment for reducing - and potentially even preventing - CMV infectious in patients eligible for organ and stem cell transplants."’
At present, there is no effective vaccine against HCMV, and anti-viral drugs often prove ineffective or have very serious side-effects. Now, in a study published in Nature Communications, researchers at Vrije Universiteit Amsterdam in the Netherlands and at the University of Cambridge have found a way to chase the virus from its hiding place using a special type of antibody known as a nanobody.
Nanobodies were first identified in camels and exist in all camelids - a family of animals that also includes dromedary, llamas and alpacas. Human antibodies consist of two heavy and two light chains of molecules, which together recognise and bind to markers on the surface of a cell or virus known as antigens. For this special class of camelid antibodies, however, only a single fragment of the antibody - often referred to as single domain antibody or nanobody - is sufficient to properly recognize antigens.
Dr Timo De Groof from Vrije Universiteit Amsterdam, the study's joint first author, said: "As the name suggests, nanobodies are much smaller than regular antibodies, which make them perfectly suited for particular types of antigens and relatively easy to manufacture and adjust. That's why they're being hailed as having the potential to revolutionise antibody therapies."
The first nanobody has been approved and introduced onto the market by biopharmaceutical company Ablynx, while other nanobodies are already in clinical trials for diseases like rheumatoid arthritis and certain cancers. Now, the team in The Netherlands and the UK have developed nanobodies that target a specific virus protein (US28), one of the few elements detectable on the surface of a HCMV latently infected cell and a main driver of this latent state.
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In laboratory experiments using blood infected with the virus, the team showed that the nanobody binds to the US28 protein and interrupts the signals established through the protein that help keep the virus in its dormant state. Once this control is broken, the local immune cells are able to 'see' that the cell is infected, enabling the host's immune cells to hunt down and kill the virus, purging the latent reservoir and clearing the blood of the virus.
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Source-Eurekalert