New Covid-19 Test Developed

Low-cost and fast method to detect a COVID-19 infection has been developed. Cap-iLAMP drastically reduces false positives and single infected samples can be detected in a pool among 25 uninfected samples, thus reducing the technical cost per test to only about 1 Euro per individual.

New coronavirus test called Colorimetric RT-LAMP assay (Cap-iLAMP) has been developed, reveals a new study.

Quantitative real-time polymerase chain reaction (qPCR) is the most widely used diagnostic method to detect RNA viruses such as SARS-CoV-2. However, it requires expensive laboratory equipment and global shortages of reagents for RNA purification has increased the need to find simple but reliable alternatives.

‘Colorimetric RT-LAMP assay (Cap-iLAMP) helps reduce false positives and single infected samples can be detected, thus reducing the technical cost per test to only about 1 Euro per individual. ’

One alternative to the qPCR technology is RT-LAMP (reverse transcription loop-mediated isothermal amplification). This test amplifies the desired target sequences of the virus at a constant temperature, using minimal equipment compared to qPCR.

In 2020, it was adapted to the detection of SARS-CoV-2. It was also shown that instead of a swab, which many people find unpleasant, it can be performed on gargle lavage samples.

First author Lukas Bokelmann and colleagues have now developed an improved colorimetric RT-LAMP assay, called Cap-iLAMP (capture and improved loop-mediated isothermal amplification), which extracts and concentrates viral RNA from a pool of gargle lavage samples.

Coronavirus
Coronaviruses infect animals, humans, and birds. Human coronaviruses, such as SARS, MERS, and 2019-nCoV cause respiratory distress and even death. The S protein is the infectious agent of the virus.

After a short incubation, the test result - orange/red for negative, bright yellow for positive - can be interpreted visually or by using a freely available smartphone app.

The improved testing method outperforms previous similar methods.

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"Cap-iLAMP drastically reduces false positives and single infected samples can be detected in a pool among 25 uninfected samples, thus reducing the technical cost per test to only about 1 Euro per individual", says senior author Stephan Riesenberg, a researcher at the Max Planck Institute for Evolutionary Anthropology.

"Our method overcomes problems associated with standard RT-LAMP and could also be applied to numerous other pathogens."

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Source-Eurekalert