New DNA Test can Rapidly Diagnose Ebola and Other Pathogens Such as Chikungunya

Researchers have developed a new DNA test that can be used to rapidly diagnose Ebola and other pathogens such as Chikungunya and hepatitis C virus.

The researchers said that the test can be used even where lab space and medical infrastructure are scarce.

US Officials To Stop Ebola Screening For Air Passengers from Liberia
The Centers of Disease Control and Prevention (CDC) has decided to remove Liberia from screening list as Ebola is no longer spreading in the country.

"This point-of-care genomic technology will be particularly attractive in the developing world, where critical resources, including reliable electric power, laboratory space, and computational server capacity, are often severely limited," said lead researcher Charles Chiu, associate professor of laboratory medicine at University of California, San Francisco, US.

The team detected the genetic fingerprints of Ebola in stored blood samples from two African patients who had acute hemorrhagic fever, completing the diagnosis within five hours of opening the samples--the DNA sequencing itself took just 10 minutes, the study said.

Most commercially available or research-based genetic diagnostic tests target specific pathogens.

New Chip-Based Technology Offers Reliable, Fast, Accurate Detection of Ebola
The newly developed system uses direct optical detection of viral molecules and can be integrated into a simple, portable instrument for use in field situations.

But the researchers have pioneered techniques that do not require suspected pathogens to be identified beforehand in order to detect their unique genetic fingerprints.

This unbiased approach of analysing all DNA in a clinical sample without knowing which species are present, which was used in the Ebola detection, is called "metagenomic" analysis.

Portable Chip-Based Technique Enables Rapid and Accurate Detection of Ebola Virus
The system combines two small chips, a microfluidic chip for sample preparation and an optofluidic chip for optical detection.

To obtain such quick results the researchers developed new analysis and visualisation software and used it on a laptop computer to leverage an emerging DNA-sequencing technology known as nanopore sequencing.

In the same set of experiments, the researchers were able to detect Chikungunya virus, from a Puerto Rican outbreak, just as quickly in a blood sample from a donor with no symptoms, but who eventually reported having fever and joint pains.

Associated Press Investigation Reveals Errors That Undermined Fight Against Ebola
The World Health Organization (WHO), charged with leading the fight against global outbreaks, already has been criticized over its management of the disease-fighting effort.

In another example of the technique's power, detection of hepatitis C virus in blood from an infected patient, present at a much lower concentration than the other viruses, took just 40 minutes from the start of sequencing.

The findings were published online in the journal Genome Medicine.

Source-IANS