Novel System Enables Large-scale Preclinical Testing of Heart Drugs & Therapies

Researchers extend life of heart segments from 24 hours to 6 days; Process allows better testing for efficacy and toxicity and saves money and time

Novel culture system that enables to keep slices of human hearts alive for a longer period of time, allowing more extensive testing of new drugs and gene therapies, was developed by the researchers at the University of Louisville.//

This new biomimetic culture system mimics the environment of a living organ through continuous electrical stimulation and oxygenation, maintaining viability and functionality of the heart segments for six days. Previous culture systems maintained functional heart slices for no more than 24 hours. The extended viability time will enable improved preclinical testing of new drugs for effectiveness and toxicity.

‘Novel biomimetic culture system helps to save time and cost of phase one clinical trials where testing for toxicity and efficacy is done. Also, the method enables to test effectiveness of gene therapy apart from drugs.’
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"This new method maintains fully functional human heart slices for six days in the culture environment. This facilitates testing efficacy of heart failure therapeutics and cardiotoxins on human heart tissue with no need for a living human," said Tamer M. A. Mohamed, Ph.D., who led the research.

The system provides access to the complete 3D multicellular system that reflects the heart's functional and structural condition in a living person.

"This system will save time and costs of clinical trials during phase one research, which includes testing for toxicity and proof of efficacy," Mohamed said. "In addition to drugs, we have demonstrated the system's effectiveness in testing gene therapy."

The optimized medium for sustaining the heart tissue slices is described in an article published online last week in Circulation Research, a publication of the American Heart Association. It will appear in the August 30, 2019, print and online issue. Along with Mohamed, the research was conducted by Qinghui Ou, B.Sc., Riham R.E. Abouleisa, Ph.D., and others at UofL, along with colleagues in California, Colorado, the United Kingdom, Germany and Egypt. UofL has a provisional patent application on this technology.

Source-Eurekalert